Pop in 12ax7 usually require no adjustment. You can get away popping in 12ax7 as phase splitter without any adjustment unless you are picky to do the AC adjustment. That would require some test equipment. As the MPX, it may need adjustment but may still work without any adjusment. Changing any tube (6ha5, 6cw4, 6hr6, 6au6) in the tuner can detune the alignment some. I've changed out some in mine but all in the front end (6ha5, 6cw4, 6hr6) with all NOS and what a difference to the better. Changing out 6gk5 should not mess anything up, it drives the relay. I've changed out some tube in my 500c some including in the tuner and MPX. It still seem to work same but seem to have better performance because of tube I put in is NOS (like new never used) and old tube were pretty worn. I haven't done any adjustment in mine but I do think it needs alignment otherwise works (been that way before retube). Messing with adjustment in tuner need skills and equipment. What I would do is pop in the new tube and do no adjusment and see how it sounds.