Wow--nice report. You are definitely at the bleeding edge. Very interesting!
Heh. It's just my rambling stream of thought thing. But thanks!
This is a great project for learning the physics of light.
I had a situation yesterday during a setup that really drove home the point regarding how light affects the position of the objective in relation to the subject you're shooting. I had set up unbalanced light (one side only).
When I went to set the start/end travel limits for the focusing rail, it looked like the fly wing was moving when looking at it in the viewfinder. The wing moved, or appeared to move entirely out of the field of view. The travel on the rail was less than a millimeter, or .04" (the field of view width is .71 mm or .028" wide -- irrelevant in this instance) . That really had me stumped. Then I positioned the light exactly where the flash head was going to be located which provided balanced lighting. This solved the problem.
What happens is the angle of the light being reflected back into the objective changes as the objective moves due to the unbalanced light. It looks like the horsefly wing is moving, but it isn't, it's the light itself that makes it look like it's moving.
Those scales on the butterfly wing are roughly .002-.004" wide. Crazy small.
I wish I'd taken a better picture of the setup. I tore it down and will be trying some other things soon enough.